Kinetics and mechanism of action of muscle pyruvate kinase
نویسندگان
چکیده
منابع مشابه
The correlation of reaction kinetics and substrate binding with the mechanism of pyruvate kinase.
Applications of isotopic techniques have contributed considerably to the understanding of kinase mechanisms. Studies with 018 (l-3) have shown that various kinase reactions proceed by transfer of a phosphoryl group without exchange of the phosphoryl oxygens with water or substrates’ Evidence that the kinase reactions do not involve formation of a phosphoryl enzyme intermediate is given by the i...
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Background: The frequency of pyruvate kinase (PK) deficiency, an autosomal recessive defect, is approximately 3 per 10,000 individuals in Shiraz and surrounding areas, and is increased due to high consanguinity marriage frequency. The purpose of this study is to obtain data on the frequency and spectrum of gene mutation of PK in newborns, from Shiraz and surrounding areas. Materials and Methods...
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Pyruvate has been shown to both stimulate and inhibit kidney pyruvate dehydrogenase, (PDH,) kinase activity. The present study investigates the inhibitory effect of pyruvate under conditions in which the stimulatory effect is invariant. Inhibition of PDH, kinase activity by dichloroacetate, a pyruvate analog, is also characterized. Both pyruvate and dichloroacetate are uncompetitive, hyperbolic...
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Pyruvate has been shown to both stimulate and inhibit kidney pyruvate dehydrogenase, (PDH,) kinase activity. The present study investigates the inhibitory effect of pyruvate under conditions in which the stimulatory effect is invariant. Inhibition of PDH, kinase activity by dichloroacetate, a pyruvate analog, is also characterized. Both pyruvate and dichloroacetate are uncompetitive, hyperbolic...
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The activation of pyruvate dehydrogenasea kinase activity by CoA esters has been further characterized. Half-maximal activation of kinase activity was achieved with about 1.0 microM acetyl-CoA after a 20-s preincubation in the presence of NADH. More than 80% of the acetyl-CoA was consumed during this period in acetylating sites in the pyruvate dehydrogenase complex as a result of the transacety...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0264-6021
DOI: 10.1042/bj1690039